This volume presents a series of protocols and methods, some of which are not widely used by researchers/practitioners, and will aid in the execution of different laboratory techniques. Forensic DNA Typing Protocols, Second Edition is arranged into a series of related chapters. Chapter 1-3 examines two different aspects of RNA analysis for body fluid identification. Chapters 4-7 focuses on the storage of biological materials and the extraction of DNA from hard tissues. Chapters 8-10 present methods for monitoring the quality of DNA extracts, and steps to aid in the purification of DNA. Chapters 11-16 talk about methods on non-standard markers, such as INDELs, Y chromosome STRs, and mitochondrial DNA. Detailed procedures and data analysis for phenotypes and ancestry are explored in Chapter 17-19. The last chapter (20) looks at the application of DNA typing to the identification of non-human material to species level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Practical and thorough, Forensic DNA Typing Protocols, Second Edition, is a valuable resource for forensic specialists, researchers, and anyone interested in the field of forensic science.

1. Collection of Samples for DNA Analysis

Roland A.H. van Oorschot, Timothy J. Verdon, and Kaye N. Ballantyne



2. Body Fluid Identification Using mRNA Profiling

Amy D. Roeder and Cordula Haas



3. mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification

Joanna Jakubowska, Agnieszka Maciejewska, and Ryszard Pawlowski



4. Preservation of and DNA Extraction from Muscle Tissue

Dennis McNevin



5. DNA Extraction: Organic and Solid-Phase

Wafa Ibrahim



6. Extraction of DNA from Skeletal Remains

Suni M. Edson and Timothy P. McMahon



7. Extraction of DNA from Human Skeletal Material

Irena Zupani c Pajnic



8.  The Development and Use of Internal Amplification Controls (IACs) with DNA Profiling Kits for Forensic DNA Analysis

Nathalie Zahra and William Goodwin



9.  NucleoSpin ®XS Columns for DNA Concentration and Clean-Up

William R. Hudlow



10. Purification of PCR Products to Improve STR Profiles

Amy D. Roeder



11. Analysis of 30 Biallelic INDEL Markers Using the Investigator DIPplex ® Kit

Majid Bashir and Nur Haliza Bt Hassan



12. Analysis of Mitochondrial Control Region Using Sanger Sequencing

David Ballard



13. Whole Human Mitochondrial DNA Sequencing

Igor V. Ovchinnikov, Mathew J. Malek, Katelyn Kjelland, and Kenneth Drees



14. In-Solution Hybridization for the Targeted Enrichment of the Whole Mitchondrial Genome

B. Bekaert, R. Ellerington, L. Van den Abbeele, and R. Decorte



15. Enhanced DNA Profiling of the Semen Donor in Late Reported Sexual Assaults: Use of Y-Chromosome Targeted Pre-Amplification and Next Generation Y-STR Amplification Systems

Erin K. Hanson and Jack Ballantyne



16. Analysis of Rapidly Mutating Y Chromosome Short Tandem Repeats (RM Y STRs)

Sibte Hadi



17. A Practical Guide to the HIrisPlex System: Simultaneous Prediction of Eye and Hair Color from DNA

Susan Walsh and Manfred Kayser



18. Inference of Ancestry in Forensic Analysis I: Autosomal Ancestry - Informative Marker Sets

Chris Phillips, Carla Santos, Manuel Fondevilla, Ángel Carracedo, and Maria Victoria Lareu



19. Inference of Ancestry in Forensic Analysis II: Analysis of Genetic Data

Carla Santos, Chris Phillips, A. Gomez-Tato, J. Alvarez-Dios, Ángel Carracedo, Maria Victoria Lareu



20. Species Determination: The Role and Use of the Cytochrome b Gene

Adrian Linacre and James Chun-I Lee