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Functional Analysis of Long­ Non-Coding RNAs
Methods and Protocols (Methods in Molecular Biology)
By Haiming Cao (Edited by)

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Format
Hardback, 362 pages
Other Formats Available

Paperback : HK$1,032.00

Published
United States, 1 December 2020


1. Bioinformatics Approaches for Functional Prediction of Long Non-Coding RNAs


Fayaz Seifuddin and Mehdi Pirooznia



2. Visualization of lncRNA and mRNA Structure Models within the Integrative Genomics Viewer


Steven Busan and Kevin M. Weeks



3. RNA Coding Potential Prediction Using Alignment-Free Logistic Regression Model


Ying Li and Liguo Wang



4. Classification of Long Non-Coding RNAs by K-mer Content


Jessime M. Kirk, Daniel Sprague, and J. Mauro Calabrese



5. Genome-Wide Computational Analysis and Validation of Potential Long Non-Coding RNA-Mediated DNA-DNA:RNA Triplexes in the Human Genome


Saakshi Jalali, Amrita Singh, Vinod Scaria, and Souvik Maiti



6. Using INFERNO to Infer the Molecular Mechanisms Underlying Non-Coding Genetic Associations


Alexandre Amlie-Wolf, Pavel P. Kuksa, Chien-Yueh Lee, Elisabeth Mlynarski, Yuk Yee Leung, and Li-San Wang



7. A Bioinformatic Pipeline to Integrate GWAS and eQTL Datasets to Identify Disease Relevant Human Long Non-Coding RNAs


Yi Chen, Ping Li, and Haiming Cao



8. AnnoLnc: A One-Stop Portal to Systematically Annotate Novel Human Long Non-Coding RNAs


De-Chang Yang, Lan Ke, Yang Ding, and Ge Gao



9. Annotation of Full-Length Long Non-Coding RNAs with Capture Long-Read Sequencing (CLS)


Silvia Carbonell Sala, Barbara Uszczynska-Ratajczak, Julien Lagarde, Rory Johnson, and Roderic Guigo



10. Single-Cell Analysis of Long Non-Coding RNAs (lncRNAs) in Mouse Brain Cells


Boyang Zhang, Wentao Xu, and James Eberwine



11. Detection and Characterization of Ribosome-Associated Long Non-Coding RNAs


Chao Zeng and Michiaki Hamada



12. Analysis of Annotated and Unannotated Long Non-Coding RNAs from Exosome Subtypes Using Next-Generation RNA Sequencing


Wittaya Suwakulsiri, Maoshan Chen, David W. Greening, Rong Xu, and Richard J. Simpson



13. DMS-MaPseq for Genome-Wide or Targeted RNA Structure Probing In Vitro and In Vivo


Phillip Tomezsko, Harish Swaminathan, and Silvi Rouskin



14. Labeling and Purification of Temporally Expressed RNAs during the S-Phase of the Cell Cycle in Living Cells


Matthieu Meryet-Figuiere, Mohamad Moustafa Ali, Santhilal Subhash, and Chandrasekhar Kanduri


15. A Quick Immuno-FISH Protocol for Detecting RNAs, Proteins, and Chromatin Modifications


Akiyo Ogawa and Yuya Ogawa



16. M(R)apping RNA-Protein Interactions


Jasmine Barra, Roberto Vendramin, and Eleonora Leucci



17. In Vivo Administration of Therapeutic Antisense Oligonucleotides


Luisa Statello, Mohamad Moustafa Ali, and Chandrasekhar Kanduri



18. CRISPR-Mediated Mutagenesis of Long Non-Coding RNAs


Tomohiro Yamazaki and Tetsuro Hirose



19. In Vivo CRISPR/Cas9-Based Targeted Disruption and Knock-In of a Long Non-Coding RNA


Xi Cheng, Samuel T. Peters, Shondra M. Pruett-Miller, Thomas Saunders, and Bina Joe



20. Genome-Scale Perturbation of Long Non-Coding RNA Expression Using CRISPR Interference


S. John Liu, Max A. Horlbeck, Jonathan S. Weissman, and Daniel A. Lim



21. In Vivo Functional Analysis of Non-Conserved Human lncRNAs Using a Humanized Mouse Model


Yonghe Ma, Cheng-fei Jiang, Ping Li, and Haiming Cao

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Product Description


1. Bioinformatics Approaches for Functional Prediction of Long Non-Coding RNAs


Fayaz Seifuddin and Mehdi Pirooznia



2. Visualization of lncRNA and mRNA Structure Models within the Integrative Genomics Viewer


Steven Busan and Kevin M. Weeks



3. RNA Coding Potential Prediction Using Alignment-Free Logistic Regression Model


Ying Li and Liguo Wang



4. Classification of Long Non-Coding RNAs by K-mer Content


Jessime M. Kirk, Daniel Sprague, and J. Mauro Calabrese



5. Genome-Wide Computational Analysis and Validation of Potential Long Non-Coding RNA-Mediated DNA-DNA:RNA Triplexes in the Human Genome


Saakshi Jalali, Amrita Singh, Vinod Scaria, and Souvik Maiti



6. Using INFERNO to Infer the Molecular Mechanisms Underlying Non-Coding Genetic Associations


Alexandre Amlie-Wolf, Pavel P. Kuksa, Chien-Yueh Lee, Elisabeth Mlynarski, Yuk Yee Leung, and Li-San Wang



7. A Bioinformatic Pipeline to Integrate GWAS and eQTL Datasets to Identify Disease Relevant Human Long Non-Coding RNAs


Yi Chen, Ping Li, and Haiming Cao



8. AnnoLnc: A One-Stop Portal to Systematically Annotate Novel Human Long Non-Coding RNAs


De-Chang Yang, Lan Ke, Yang Ding, and Ge Gao



9. Annotation of Full-Length Long Non-Coding RNAs with Capture Long-Read Sequencing (CLS)


Silvia Carbonell Sala, Barbara Uszczynska-Ratajczak, Julien Lagarde, Rory Johnson, and Roderic Guigo



10. Single-Cell Analysis of Long Non-Coding RNAs (lncRNAs) in Mouse Brain Cells


Boyang Zhang, Wentao Xu, and James Eberwine



11. Detection and Characterization of Ribosome-Associated Long Non-Coding RNAs


Chao Zeng and Michiaki Hamada



12. Analysis of Annotated and Unannotated Long Non-Coding RNAs from Exosome Subtypes Using Next-Generation RNA Sequencing


Wittaya Suwakulsiri, Maoshan Chen, David W. Greening, Rong Xu, and Richard J. Simpson



13. DMS-MaPseq for Genome-Wide or Targeted RNA Structure Probing In Vitro and In Vivo


Phillip Tomezsko, Harish Swaminathan, and Silvi Rouskin



14. Labeling and Purification of Temporally Expressed RNAs during the S-Phase of the Cell Cycle in Living Cells


Matthieu Meryet-Figuiere, Mohamad Moustafa Ali, Santhilal Subhash, and Chandrasekhar Kanduri


15. A Quick Immuno-FISH Protocol for Detecting RNAs, Proteins, and Chromatin Modifications


Akiyo Ogawa and Yuya Ogawa



16. M(R)apping RNA-Protein Interactions


Jasmine Barra, Roberto Vendramin, and Eleonora Leucci



17. In Vivo Administration of Therapeutic Antisense Oligonucleotides


Luisa Statello, Mohamad Moustafa Ali, and Chandrasekhar Kanduri



18. CRISPR-Mediated Mutagenesis of Long Non-Coding RNAs


Tomohiro Yamazaki and Tetsuro Hirose



19. In Vivo CRISPR/Cas9-Based Targeted Disruption and Knock-In of a Long Non-Coding RNA


Xi Cheng, Samuel T. Peters, Shondra M. Pruett-Miller, Thomas Saunders, and Bina Joe



20. Genome-Scale Perturbation of Long Non-Coding RNA Expression Using CRISPR Interference


S. John Liu, Max A. Horlbeck, Jonathan S. Weissman, and Daniel A. Lim



21. In Vivo Functional Analysis of Non-Conserved Human lncRNAs Using a Humanized Mouse Model


Yonghe Ma, Cheng-fei Jiang, Ping Li, and Haiming Cao

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Product Details
EAN
9781071611579
ISBN
1071611577
Other Information
54 Tables, color; 54 Illustrations, color; 9 Illustrations, black and white; X, 362 p. 63 illus., 54 illus. in color.
Dimensions
26.2 x 19.8 x 2.3 centimeters (0.89 kg)

Table of Contents

Bioinformatics Approaches for Functional Prediction of Long Non-Coding RNAs.- Visualization of lncRNA and mRNA Structure Models within the Integrative Genomics Viewer.- RNA Coding Potential Prediction Using Alignment-Free Logistic Regression Model.- Classification of Long Non-Coding RNAs by K-mer Content.- Genome-Wide Computational Analysis and Validation of Potential Long Non-Coding RNA-Mediated DNA-DNA:RNA Triplexes in the Human Genome.- Using INFERNO to Infer the Molecular Mechanisms Underlying Non-Coding Genetic Associations.- A Bioinformatic Pipeline to Integrate GWAS and eQTL Datasets to Identify Disease Relevant Human Long Non-Coding RNAs.- AnnoLnc: A One-Stop Portal to Systematically Annotate Novel Human Long Non-Coding RNAs.- Annotation of Full-Length Long Non-Coding RNAs with Capture Long-Read Sequencing (CLS).- Single-Cell Analysis of Long Non-Coding RNAs (lncRNAs) in Mouse Brain Cells.- Detection and Characterization of Ribosome-Associated Long Non-Coding RNAs.- Analysis ofAnnotated and Unannotated Long Non-Coding RNAs from Exosome Subtypes Using Next-Generation RNA Sequencing.- DMS-MaPseq for Genome-Wide or Targeted RNA Structure Probing In Vitro and In Vivo.- Labeling and Purification of Temporally Expressed RNAs during the S-Phase of the Cell Cycle in Living Cells.- A Quick Immuno-FISH Protocol for Detecting RNAs, Proteins, and Chromatin Modifications.- M(R)apping RNA-Protein Interactions.- In Vivo Administration of Therapeutic Antisense Oligonucleotides.- CRISPR-Mediated Mutagenesis of Long Non-Coding RNAs.- In Vivo CRISPR/Cas9-Based Targeted Disruption and Knock-In of a Long Non-Coding RNA.- Genome-Scale Perturbation of Long Non-Coding RNA Expression Using CRISPR Interference.- In Vivo Functional Analysis of Non-Conserved Human lncRNAs Using a Humanized Mouse Model.

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