Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. * Volume of Methods In Enzymology* Contains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization
Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. * Volume of Methods In Enzymology* Contains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization
This volume of Methods in Enzymology provides a unique platform for the researchers interested in general area of membrane protein structure and function
Section I: Recombinant Expression of Membrane Proteins
1. Engineering Escherichia Coli for Functional Expression of
Membrane Proteins
Franz Y Ho and Bert Poolman
2. ACEMBLing a Multiprotein Transmembrane Complex: The
Functional SecYEG-SecDFYajC-YidC Holotranslocon Protein
Secretase/Insertase
Joanna Komar, Mathieu Botte, Ian Collinson, Christiane Schaffitzel
and Imre Berger
3. Expression and Purification of OsVDAC4
Ashwini Godbole, Ashvini K. Dubey, Rohan Mitra, Palakolanu S. Reddy
and M. K. Mathew
4. Membrane Protein Expression in Lactococcus Lactis
Martin S. King, Christoph Boes and Edmund R.S. Kunji
5. An Unconventional Anaerobic Membrane Protein Production
System Based on Wolinella Succinogenes
Michael Lafontaine and C. Roy D. Lancaster
6. Membrane Protein Expression and Analysis in Yeast
Katrien Claes, Mouna Guerfal and Nico Callewaert
7. Heterologous Expression of G Protein Coupled Receptors in
Yeast
Nicolas Bertheleme, Shweta Singh, Simon Dowell and Bernadette
Byrne
8. Recombinant G protein-Coupled Receptor Expression in
Saccharomyces Cerevisiae for Protein Characterization
Kory M. Blocker, Zachary T. Britton, Andrea N. Naranjo, Patrick M.
McNeely, Carissa L. Young and Anne S. Robinson
9. Baculovirus Mediated Expression of GPCRs in Insect
Cells
Tuulia Saarenpää, Veli-Pekka Jaakola and Adrian Goldman
10. Expression of Membrane Proteins in the Eyes of Transgenic
Drosophila Melanogaster
Yvonne Hackmann, Lisa Joedicke, Valérie Panneels and Irmgard
Sinning
11. Rapid Method to Express and Purify Human Membrane Protein
Using the Xenopus Oocyte System for Functional and Low-Resolution
Structural Analysis
Benjamin Clémençon, Michael Fine, Philipp Schneider and Matthias A.
Hediger
12. Expression of G protein-coupled Receptors in Mammalian
Cells
Raja Chakraborty, Bing Xu, Rajinder P. Bhullar and Prashen
Chelikani
13. Construction of Stable Mammalian Cell Lines for Inducible
Expression of G Protein-Coupled Receptors
Chikwado A. Opefi, Dale Tranter, Steven O. Smith and Philip J.
Reeves.
14. Rapid and Facile Recombinant Expression of Bovine
Rhodopsin in HEK293S GnTI– Cells Using a PiggyBac Inducible
System
Lydia N. Caro, Zhijie Li, James M. Rini, Aidin R. Balo and Oliver
P. Ernst
15. Semliki Forest Virus-based Expression of Recombinant
GPCRs
Kenneth Lundstrom
16. Screening for Lipid Requirements of Membrane Proteins by
Combining Cell-Free Expression with Nanodiscs
Erik Henrich, Volker Dötsch, and Frank Bernhard
Section II. Reconstitution and Functional Characterization of
Membrane Proteins
17. Liposome Reconstitution and Transport Assay for
Recombinant Transporters
Zachary Lee Johnson and Seok-Yong Lee
18. Lipid Reconstitution and Recording of Recombinant Ion
Channels
Randy Stockbridge and Ming-Feng Tsai
19. Reconstitution of Membrane Proteins - a GPCR as an
Example
Alan D. Goddard, Patricia M. Dijkman, Roslin J. Adamson, Rosana
Inácio dos Reis and Anthony Watts
20. Ion Channel Reporter for Monitoring the Activity of
Engineered GPCRs
Christophe J. Moreau, Katarzyna Niescierowicz , Lydia Caro ,
Jean Revilloud and Michel Vivaudou
21. Methods for Labeling Skeletal Muscle Ion Channels
Site-Specifically With Fluorophores Suitable for FRET-based
Structural Analysis
Mohana Mahalingam and James D. Fessenden
22. Structural and Functional Studies of Nirc From Salmonella
Typhimurium
Adriana Rycovska-Blume,Wei Lü, Susana Andrade, Klaus Fendler and
Oliver Einsle
23. Surface Plasmon Resonance Analysis of 7-Transmembrane
Receptors
Tonia Aristotelous, Andrew L. Hopkins and Iva Navratilova
24. Crosslinking Strategies to Study Peptide Ligand-Receptor
Interactions
Jeffery Becker
25. From recombinant expression to crystals – A step-by-step
guide to GPCR crystallography
Arun K. Shukla, Punita Kumari, Eshan Ghosh and Kumari
Nidhi
26. Structure-based Biophysical Analysis of the Interaction of
Rhodopsin with G Protein and Arrestin
Martha E. Sommer, Matthias Elgeti, Peter W. Hildebrand, Michal
Szczepek, Klaus Peter Hofmann and Patrick Scheerer
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.
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