Nanobiotechnology holds the promise of providing revolutionary insight into aspects biology ranging from fundamental questions such as elucidating molecular mechanisms of brain disorders to extraordinary applications such as the detection of a single cancer cell in a population of a million cells.  The second edition of Nanobiotechnology Protocols expands upon the previous editions with current, detailed protocols for nanobiotechnology; imaging and detection. With new chapters that explore nano-bio constructs and toxicology of nanomaterials. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Nanobiotechnology Protocol, Second Edition highlights important current areas of development and directions of the field for the future.

Authors Sandra J. Rosenthal and David W. Wright

1. High Aspect Ratio Gold Nanorods: Their Synthesis and Application to Image Cell-Induced Strain Fields in Collagen Films

Davin J. Chernak,  Patrick N. Sisco, Edie C. Goldsmith, Sarah C. Baxter, and Catherine J. Murphy

2. Imaging Intracellular Quantum Dots: Fluorescence Microscopy and Transmission Electron Microscopy

Craig J. Szymanski, Hong Yi, Joshua L. Liu, Elizabeth R. Wright, and Christine K. Payne

3. Imaging of Cell Populations in Atherosclerosis Using Quantum Dot Nanocrystals

Joshua R. Trantum and Ashwath Jayagopal

4. Imaging of Endothelial Progenitor Cell Subpopulations in Angiogenesis using Quantum Dot Nanocrystals

Joshua M. Barnett, John S. Penn and Ashwath Jayagopal

5. Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots

Qi Zhang

6. Quantum Dot-Based Single-Molecule Microscopy for the Study of Protein Dynamics

Jerry C. Chang and Sandra J. Rosenthal

7. Three-dimensional Molecular Imaging with Photothermal Optical Coherence Tomography

Melissa C. Skala, Matthew J. Crow, Adam Wax and Joseph A. Izatt

8. Detecting Respiratory Syncytial Virus Using Nanoparticle Amplified Immuno-PCR

Jonas W. Perez, Nicholas M. Adams, Grant R. Zimmerman, Frederick R. Haselton and David W. Wright

9. Gold Nanoparticle-Oligonucleotide Conjugates for the Profiling of Malignant Melanoma Phenotypes

John W. Stone, Reese Harry, Owen Hendley and David W. Wright

10. Methods for Isolating RNA Sequences Capable of Binding to or Mediating the Formation of Inorganic Materials

Carly Jo Carter, Alina Owczarek and Daniel L. Feldheim

11. Single-Walled Carbon Nanotube-Mediated Small Interfering RNA Delivery and Silencing Gastrin-Releasing Peptide Receptor in Human Neuroblastoma Cells

Jingbo Qiao, Honglian Guo, Ya-Qiong Xu and Dai H. Chung

12. Amino Acid Mediated Linear Assembly of Au Nanomaterials

Manish Sethi and Marc R. Knecht

13. Enzyme-Gold Nanoparticle Bioconjugates: Quantification of Particle Stoichiometry and Enzyme Specific Activity

Jacqueline D. Keighron and Christine D. Keating

14. In Vivo Testing for Gold Nanoparticle Toxicity

Carrie A. Simpson, Brian J. Huffman, and David E. Cliffel

15. Methods for Studying of Toxicity of Silica-based Nanomaterials to Living Cells

Yang Zhao, Yuhui Jin, Aaron Hanson, Min Wu and Julia Xiaojun Zhao