Preface xiii
Preparation for Protein Isolation 1
Protein Extraction and Solubilization 27
Protein Concentration Determination 57
Concentrating Protein Solutions 83
Gel Electrophoresis Under Denaturing Conditions 107
Gel Electrophoresis Under Nondenaturing Conditions 155
Isoelectric Focusing and Two-Dimensional Gel Electrophoresis
173
Immunoblotting 195
Ion Exchange Chromatography 231
Gel Filtration Chromatography 271
Affinity Chromatography 301
Hanging Drop Crystallization 353
Appendix 1: Molecular weights of commonly used Chemicals 389
Appendix 2: Molecular weights and Isoelectric Points of Selected Protiens 393
Appendix 3: Ammonium Sulfate Precipitation Table 394
Appendix 4: Spectrophotometer Linearity 397
Appendix 5: Suppliers and Addressed 399
Index 405
Daniel M. Bollag and Michael D. Rozycki are the authors of Protein Methods, 2nd Edition, published by Wiley.
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