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Staphylococcus aureus
Methods and Protocols (Methods in Molecular Biology)
By Kelly C. Rice (Edited by)

Rating
Format
Paperback, 162 pages
Other Formats Available

Hardback : HK$1,277.00

Published
United States, 1 July 2022


Preface...

Table of Contents...

Contributing Authors...


1. Measurement of Staphylococcus aureus Pigmentation by Methanol Extraction

Leia E. Sullivan and Kelly C. Rice


2. Analysis of Murein Hydrolases and Proteases through Zymography

Nichole A. Seawell and Jeffrey L. Bose


3. Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture Supernatants

Richard E. Wiemels, Rebecca A. Keogh, and Ronan K. Carroll


4. Quantitative Hemolysis Assays

Miranda J. Ridder, Seth M. Daly, Pamela R. Hall, and Jeffrey L. Bose


5. In Vitro Assay for Quantifying Clumping of Staphylococcus aureus

Heidi A. Crosby, Jakub M. Kwiecinski, and Alexander R. Horswill


6. Measuring Staphylococcal Promoter Activities using a Codon-Optimized ß-galactosidase Reporter

Christina N. Krute, Nichole A. Seawell, and Jeffrey L. Bose

7. Evaluation of Staphylococcus aureus Antibiotic Tolerance using Kill Curve Assays

Jessica N. Brandwein and Kelly C. Rice


8. Fluorescence Polarization (FP) Assay for Measuring Staphylococcus aureus Membrane Fluidity

Kiran B. Tiwari, Suranjana Sen, Craig Gatto, and Brian J. Wilkinson


9. Quantification of Staphylococcus aureus Biofilm Formation by Crystal

Violet and Confocal Microscopy

Adam B. Grossman, Dylan J. Burgin, and Kelly C. Rice


10. Growth of Staphylococcus aureus using a Rotary Cell Culture System

Matthew R. Hauserman and Kelly C. Rice

11. Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in Staphylococcus aureus

Logan L. Bulock, Jennifer L. Endres, and Marat R. Sadykov


12. Quantifying Staphylococcus aureus Membrane Potential using Flow Cytometry

Neal D. Hammer

13. Best Practices for Preparation of Staphylococcus aureus Metabolomics Samples

Kimberly L. James and Kelly C. Rice


14. A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm Proteins

Mei G. Lei, Ravi Kr. Gupta, and Chia Y. Lee


15. An Ex Vivo Model for Assessing Growth and Survivability of Staphylococcus aureus in Whole Human Blood

Brittney D. Gimza, Stephanie M. Marroquin, and Lindsey N. Shaw


16. Bone Marrow-Derived Macrophage Infection Assay

Miranda J. Ridder, Mary A. Markiewicz, and Jeffrey L. Bose


17. Measurement of Osteoblast Cytotoxicity Induced by S. aureus Secreted Toxins

Caleb Ford and James E. Cassat


18. Human Skin In Vitro Colonization Model for a Skin Wound Infected by Staphylococcus aureus Biofilm

Jenelle Chapman and Michael E. Olson


Subject Index List...

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Product Description


Preface...

Table of Contents...

Contributing Authors...


1. Measurement of Staphylococcus aureus Pigmentation by Methanol Extraction

Leia E. Sullivan and Kelly C. Rice


2. Analysis of Murein Hydrolases and Proteases through Zymography

Nichole A. Seawell and Jeffrey L. Bose


3. Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture Supernatants

Richard E. Wiemels, Rebecca A. Keogh, and Ronan K. Carroll


4. Quantitative Hemolysis Assays

Miranda J. Ridder, Seth M. Daly, Pamela R. Hall, and Jeffrey L. Bose


5. In Vitro Assay for Quantifying Clumping of Staphylococcus aureus

Heidi A. Crosby, Jakub M. Kwiecinski, and Alexander R. Horswill


6. Measuring Staphylococcal Promoter Activities using a Codon-Optimized ß-galactosidase Reporter

Christina N. Krute, Nichole A. Seawell, and Jeffrey L. Bose

7. Evaluation of Staphylococcus aureus Antibiotic Tolerance using Kill Curve Assays

Jessica N. Brandwein and Kelly C. Rice


8. Fluorescence Polarization (FP) Assay for Measuring Staphylococcus aureus Membrane Fluidity

Kiran B. Tiwari, Suranjana Sen, Craig Gatto, and Brian J. Wilkinson


9. Quantification of Staphylococcus aureus Biofilm Formation by Crystal

Violet and Confocal Microscopy

Adam B. Grossman, Dylan J. Burgin, and Kelly C. Rice


10. Growth of Staphylococcus aureus using a Rotary Cell Culture System

Matthew R. Hauserman and Kelly C. Rice

11. Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in Staphylococcus aureus

Logan L. Bulock, Jennifer L. Endres, and Marat R. Sadykov


12. Quantifying Staphylococcus aureus Membrane Potential using Flow Cytometry

Neal D. Hammer

13. Best Practices for Preparation of Staphylococcus aureus Metabolomics Samples

Kimberly L. James and Kelly C. Rice


14. A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm Proteins

Mei G. Lei, Ravi Kr. Gupta, and Chia Y. Lee


15. An Ex Vivo Model for Assessing Growth and Survivability of Staphylococcus aureus in Whole Human Blood

Brittney D. Gimza, Stephanie M. Marroquin, and Lindsey N. Shaw


16. Bone Marrow-Derived Macrophage Infection Assay

Miranda J. Ridder, Mary A. Markiewicz, and Jeffrey L. Bose


17. Measurement of Osteoblast Cytotoxicity Induced by S. aureus Secreted Toxins

Caleb Ford and James E. Cassat


18. Human Skin In Vitro Colonization Model for a Skin Wound Infected by Staphylococcus aureus Biofilm

Jenelle Chapman and Michael E. Olson


Subject Index List...

Show more
Product Details
EAN
9781071615522
ISBN
1071615521
Other Information
25 Illustrations, color; 9 Illustrations, black and white; X, 162 p. 34 illus., 25 illus. in color.
Dimensions
25.4 x 17.8 x 0.9 centimeters (0.34 kg)

Table of Contents

Measurement of Staphylococcus aureus Pigmentation by Methanol Extraction.- Analysis of Murein Hydrolases and Proteases through Zymography.- Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture Supernatants.- Quantitative Hemolysis Assays.- In Vitro Assay for Quantifying Clumping of Staphylococcus aureus.- Measuring Staphylococcal Promoter Activities using a Codon-Optimized β-galactosidase Reporter.- Evaluation of Staphylococcus aureus Antibiotic Tolerance using Kill Curve Assays.- Fluorescence Polarization (FP) Assay for Measuring Staphylococcus aureus Membrane Fluidity.- Quantification of Staphylococcus aureus Biofilm Formation by Crystal Violet and Confocal Microscopy.- Growth of Staphylococcus aureus using a Rotary Cell Culture System.- Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in Staphylococcus aureus.- Quantifying Staphylococcus aureus Membrane Potentialusing Flow Cytometry.- Best Practices for Preparation of Staphylococcus aureus Metabolomics Samples.- A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm Proteins.- An Ex Vivo Model for Assessing Growth and Survivability of Staphylococcus aureus in Whole Human Blood.- Bone Marrow-Derived Macrophage Infection Assay.- Measurement of Osteoblast Cytotoxicity Induced by S. aureus Secreted Toxins.- Human Skin In Vitro Colonization Model for a Skin Wound Infected by Staphylococcus aureus Biofilm.







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